European Journal of Environmental Sciences nabízí původní recenzované články z oblasti environmentálních věd, mnohdy inter- a transdisciplinární povahy se zvláštním zřetelem na evropské problémy. Časopis rovněž publikuje kritické shrnující texty a přehledy situace v oblasti ochrany životního prostředí v konkrétních regionech a zemích. Zaměřuje se na širokou škálu témat, jako například přímé a nepřímé interakce živých a neživých složek životního prostředí, interakce s lidskou společností nebo environmentální udržitelnost.


How different reproduction protocols can affect the germination of seeds: The case of three stenoendemic species on Mt. Olympus (NC Greece)

Georgios Varsamis, Theodora Merou, Katerina Tseniklidou, Katerina Goula, Spyros Tsiftsis

zveřejněno: 30. 06. 2023


Mt. Olympus, the highest mountain in Greece, is a biosphere reserve and a magnet for countless visitors. In the wider area of Olympus, at least 1,700 species and subspecies of plants are recorded, 26 of which are endemic. Most of the endemics only occur in the high or subalpine zone, which is expected to be strongly affected by climate change or in specific microsites that might be affected by several other factors. Thus, the unique flora of the mountain will probably become extinct. Ex situ conservation can be utilized to prevent and even reverse this trend and preserve plant diversity for future generations. The aim of the present study was to develop reproduction protocols for the endemic species on Mt. Olympus, Centaurea incompleta, Centaurea litochorea and Viola striis-notata, to facilitate their mass production, either for ex situ conservation or reintroduction into their natural habitats, if necessary. Seeds of the target species were collected in summer 2021. In a sample of the collected seeds, the embryo viability was checked using sequentially 1% w/v tetrazolium solution and Evans blue solution concentration of 0.25% w/v. As for the germination tests, two treatments were used to terminate seed dormancy: (a) cold stratification at } 2 °C, and (b) imbibition in gibberellic acid (250 ppm) for 48 hours. The results showed that more than 75% of the embryos in the fertile seeds were viable. In the seed germination tests, treatment with gibberellic acid resulted in germination percentages for Centaurea incompleta and Viola striis-notata are equal to or very close to the seed viability percentage. In contrast, no treatment was successful for Centaurea litochorea, as the control germination percentage was higher.

klíčová slova: endemics; gibberellic acid; plants; seed germination; stratification; viability test

Creative Commons License
How different reproduction protocols can affect the germination of seeds: The case of three stenoendemic species on Mt. Olympus (NC Greece) is licensed under a Creative Commons Attribution 4.0 International License.

210 x 297 mm
vychází: 2 x ročně
cena tištěného čísla: 150 Kč
ISSN: 1805-0174
E-ISSN: 2336-1964

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