ACTA MEDICA, Vol 62 No 4 (2019), 170–173
Ultrashort ssDNA in Retinoblastoma Patients Blood Plasma Detected by a Novel High Resolution HPCL Technique: a Preliminary Report
Kirill V. Ermakov, Alexander A. Bukhvostov, Alexander S. Vedenkin, Sergey V. Stovbun, Anton S. Dvornikov, Dmitry A. Kuznetsov
zveřejněno: 10. 02. 2020
A significant population of ultrashort (50–150n) single-stranded DNA fragments were found in exosome-free blood plasma of retinoblastoma patients (6.84 ng mL−1), but not in plasma of healthy donors. An original high resolution HPLC technique has been proposed to reveal and characterize this peculiarity. To solve this task, a novel molecular size exclusion – anion exchange analytical technique was developed. Its applicability to diagnostics and oncogenesis research is quizzed here.
Klíčová slova: Retinoblastoma; blood plasma; cfDNA; ssDNA; HPLC
Ultrashort ssDNA in Retinoblastoma Patients Blood Plasma Detected by a Novel High Resolution HPCL Technique: a Preliminary Report is licensed under a Creative Commons Attribution 4.0 International License.