PRAGUE MEDICAL REPORT
PRAGUE MEDICAL REPORT
Prague Medical Report is an English quarterly published multidisciplinary biomedical journal. Prague Medical Report was founded as Sborník lékařský in May 1885. The journal presents public primary scientific publications, short communications, casuistry, and reviews. It contains articles based on important specialised lectures and symposia.

PRAGUE MEDICAL REPORT, Vol 119 No 1 (2018), 61–69

Identification of Etiologic Agents of the Pertussis-like Syndrome in Children by Real-time PCR Method

Shima Mahmoudi, Maryam Banar, Babak Pourakbari, Hediyeh Sadat Alavi, Hamid Eshaghi, Alireza Aziz Ahari, Setareh Mamishi

DOI: https://doi.org/10.14712/23362936.2018.6
published online: 18. 04. 2018

abstract

The aim of this study was to recognize the identity and frequency of etiologic agents of the pertussis-like syndrome in children < 2 years of age. A cross-sectional hospital-based study conducted from August 2014 to August 2015. All children < 2 years of age (n=100) who were suspected as pertussis infected were enrolled in this study and tested for Bordetella pertussis, adenovirus (Adv), respiratory syncytial virus (RSV), human metapneumovirus (hMPV), and influenza virus A (INF-A) by real-time PCR technique. RSV was the most detected pathogen (20%), followed by B. pertussis (18%), Adv (16%), INF-A (11%), and hMPV (10%). Co-infection was observed in 8 patients (11%) and the combinations of RSV/INF-A (n=3, 4%), and AdV/B. pertussis (n=3, 4%) were more frequent. RSV, B. pertussis, and hMPV were more frequent pathogens among infants < 4 months of age. However, Adv and INF-A were more frequent pathogens among children > 6 months of age. In this study, RSV was the most frequent identified pathogen (n=20, 20%), followed by B. pertussis (n=18, 18%) and AdV (n=16, 16%). Pertussis was more frequent in spring (8%) and summer (6%). In addition, clinical symptoms of pertussis were the same as some viral pathogens, which can lead to misdiagnosis of infection. Therefore, diagnosis of pertussis should be established on the bases of both the clinical symptoms and the laboratory methods.

keywords: Pertussis-like syndrome; B. pertussis; Adenovirus; Respiratory syncytial virus; Human metapneumovirus; Influenza virus A

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Identification of Etiologic Agents of the Pertussis-like Syndrome in Children by Real-time PCR Method is licensed under a Creative Commons Attribution 4.0 International License.

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ISSN: 1214-6994
E-ISSN: 2336-2936

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