EUROPEAN JOURNAL OF ENVIRONMENTAL SCIENCES
EUROPEAN JOURNAL OF ENVIRONMENTAL SCIENCES
The European Journal of Environmental Sciences offers a mixture of original peer-reviewed research papers, which bring you some of the most exciting developments in environmental sciences in the broadest sense, often with an inter- or trans-disciplinary perspective, focused on the European problems. The journal also includes critical reviews on topical issues, and overviews of the status of environmental protection in particular regions or countries. The journal covers a broad range of topics, including direct or indirect interactions between abiotic or biotic components of the environment, interactions of environment with human society, or environmental sustainability.

EUROPEAN JOURNAL OF ENVIRONMENTAL SCIENCES, Vol 2 No 1 (2012), 33–42

In vitro symbiotic seed germination and molecular characterization of associated endophytic fungi in a commercially important and endangered Indian orchid Vanda coerulea Griff. Ex Lindl.

Simmi Aggarwal, C. Nirmala, Shanuja Beri, Sachin Rastogi, Alok Adholeya

DOI: https://doi.org/10.14712/23361964.2015.36
published online: 10. 09. 2012

abstract

The technique of symbiotic seed germination-using fungi to cultivate orchid seedlings in vitro leading to their reintroduction in situ has considerable potential for conservation as evidenced by studies mostly in Australia and North America. However, its use has yet to be fully realized throughout the world. On the Indian subcontinent, which harbors a considerable number of orchid species, symbiotic germination has been virtually unexplored. In the present studies, we provide a protocol for the symbiotic seed germination and ecorestoration of an endangered orchids Vanda coerulea Griff. ex Lindl., which is a floriculturally significant epiphyte used to progenate a vast variety of hybrids. Seeds were obtained from the mature un-dehisced capsules and sown on oat meal agar medium with the fungus isolated from the roots of mature V. coerulea plants in situ. Using molecular characterization techniques, cultures were assignable to Rhizoctonia zeae with teleomorph Thanatephorus cucumeris. All the seeds germinated within 5 wks of culture and very healthy, dark green protocorms were obtained in 5 month old cultures. Seedlings with 1–2 roots and 2–3 leaves were obtained in 8 months. These were acclimatized in the greenhouse for a year and introduced to their natural habitat at Manipur in North East India. Seedling growth and development was continuously monitored, demonstrating active growth during monsoon season (April–July). Out of 29 plants reintroduced, 23 survived and are growing well with the formation of new roots and leaves, observed after twelve months of reintroduction.

keywords: Vanda coerulea; symbiotic seed germination; Rhizoctonia zeae; endangered orchid; reintroduction; molecular identification; Thanatephorus cucumeris

Creative Commons License
In vitro symbiotic seed germination and molecular characterization of associated endophytic fungi in a commercially important and endangered Indian orchid Vanda coerulea Griff. Ex Lindl. is licensed under a Creative Commons Attribution 4.0 International License.

210 x 297 mm
periodicity: 2 x per year
print price: 150 czk
ISSN: 1805-0174
E-ISSN: 2336-1964

Download