The European Journal of Environmental Sciences offers a mixture of original peer-reviewed research papers, which bring you some of the most exciting developments in environmental sciences in the broadest sense, often with an inter- or trans-disciplinary perspective, focused on the European problems. The journal also includes critical reviews on topical issues, and overviews of the status of environmental protection in particular regions or countries. The journal covers a broad range of topics, including direct or indirect interactions between abiotic or biotic components of the environment, interactions of environment with human society, or environmental sustainability.


How different reproduction protocols can affect the germination of seeds: The case of three stenoendemic species on Mt. Olympus (NC Greece)

Georgios Varsamis, Theodora Merou, Katerina Tseniklidou, Katerina Goula, Spyros Tsiftsis

published online: 30. 06. 2023


Mt. Olympus, the highest mountain in Greece, is a biosphere reserve and a magnet for countless visitors. In the wider area of Olympus, at least 1,700 species and subspecies of plants are recorded, 26 of which are endemic. Most of the endemics only occur in the high or subalpine zone, which is expected to be strongly affected by climate change or in specific microsites that might be affected by several other factors. Thus, the unique flora of the mountain will probably become extinct. Ex situ conservation can be utilized to prevent and even reverse this trend and preserve plant diversity for future generations. The aim of the present study was to develop reproduction protocols for the endemic species on Mt. Olympus, Centaurea incompleta, Centaurea litochorea and Viola striis-notata, to facilitate their mass production, either for ex situ conservation or reintroduction into their natural habitats, if necessary. Seeds of the target species were collected in summer 2021. In a sample of the collected seeds, the embryo viability was checked using sequentially 1% w/v tetrazolium solution and Evans blue solution concentration of 0.25% w/v. As for the germination tests, two treatments were used to terminate seed dormancy: (a) cold stratification at } 2 °C, and (b) imbibition in gibberellic acid (250 ppm) for 48 hours. The results showed that more than 75% of the embryos in the fertile seeds were viable. In the seed germination tests, treatment with gibberellic acid resulted in germination percentages for Centaurea incompleta and Viola striis-notata are equal to or very close to the seed viability percentage. In contrast, no treatment was successful for Centaurea litochorea, as the control germination percentage was higher.

keywords: endemics; gibberellic acid; plants; seed germination; stratification; viability test

Creative Commons License
How different reproduction protocols can affect the germination of seeds: The case of three stenoendemic species on Mt. Olympus (NC Greece) is licensed under a Creative Commons Attribution 4.0 International License.

210 x 297 mm
periodicity: 2 x per year
print price: 150 czk
ISSN: 1805-0174
E-ISSN: 2336-1964