ACTA MEDICA, Vol 56 No 2 (2013), 73–79
Effect of Collagen I Gel on Apoptosis of Rat Hepatic Stellate Cells
Lenka Bittnerová, Alena Jiroutová, Emil Rudolf, Martina Řezáčová, Jiří Kanta
DOI: https://doi.org/10.14712/18059694.2014.27
published online: 23. 07. 2015
abstract
Activated hepatic stellate cells (HSC) are a major source of fibrous proteins in cirrhotic liver. Inducing or accelerating their apoptosis is a potential way of liver fibrosis treatment. Extracellular matrix (ECM) surrounding cells in tissue affects their differentiation, migration, proliferation and function. Type I collagen is the main ECM component in fibrotic liver. We have examined how this protein modifies apoptosis of normal rat HSC induced by gliotoxin, cycloheximide and cytochalasin D in vitro and spontaneous apoptosis of HSC isolated from CCl4-damaged liver. We have found that type I collagen gel enhances HSC apoptosis regardless of the agent triggering this process.
keywords: Hepatic stellate cells; Collagen I; Apoptosis; Gliotoxin; Cykloheximide; Cytochalasin D
Effect of Collagen I Gel on Apoptosis of Rat Hepatic Stellate Cells is licensed under a Creative Commons Attribution 4.0 International License.
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ISSN: 1211-4286
E-ISSN: 1805-9694